Hotspot

psbA-matK

LSC · 1371-1994 · 624 bp

Back to Plicosepalus

Pi 0.0883

MarkerSeek score 84.10

Primer available yes

Label psbA-matK

Feature Metadata

label_name: psbA-matK
feature_type: igs
region: LSC
length_bp: 624
variable_sites: 50
pi: 0.0883
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 84.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-matK_p1	GGCTTTCACTTTTGCGTCTC	AGAAGGTCGTATTTGGTATTTGGA	50.0	37.5	58.0	57.9	685-722 bp	1.0	56.9
psbA-matK_p2	CCACAGGCTTTCACTTTTGC	AGAAGGTCGTATTTGGTATTTGGA	50.0	37.5	58.1	57.9	690-727 bp	1.0	56.0
psbA-matK_p3	CAGGCTTTCACTTTTGCGTC	AGAAGGTCGTATTTGGTATTTGGA	50.0	37.5	58.2	57.9	687-724 bp	1.0	55.3
psbA-matK_p4	AGGCTTTCACTTTTGCGTCT	AGAAGGTCGTATTTGGTATTTGGA	45.0	37.5	58.3	57.9	686-723 bp	1.0	54.7
psbA-matK_p5	GGCTTTCACTTTTGCGTCTC	GAGAAGGTCGTATTTGGTATTTGG	50.0	41.7	58.0	57.6	686-723 bp	1.0	54.5