primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psbA-matK_p1	psbA-matK	1	GGCTTTCACTTTTGCGTCTC	AGAAGGTCGTATTTGGTATTTGGA	20	24	50.0	37.5	58.0	57.9	0.0	0.0	4.095261	1371	1994	685-722	1.000000	56.9
psbA-matK_p2	psbA-matK	2	CCACAGGCTTTCACTTTTGC	AGAAGGTCGTATTTGGTATTTGGA	20	24	50.0	37.5	58.1	57.9	0.0	0.0	4.200519	1371	1994	690-727	1.000000	56.0
psbA-matK_p3	psbA-matK	3	CAGGCTTTCACTTTTGCGTC	AGAAGGTCGTATTTGGTATTTGGA	20	24	50.0	37.5	58.2	57.9	0.0	0.0	4.298538	1371	1994	687-724	1.000000	55.3
psbA-matK_p4	psbA-matK	4	AGGCTTTCACTTTTGCGTCT	AGAAGGTCGTATTTGGTATTTGGA	20	24	45.0	37.5	58.3	57.9	0.0	0.0	4.376983	1371	1994	686-723	1.000000	54.7
psbA-matK_p5	psbA-matK	5	GGCTTTCACTTTTGCGTCTC	GAGAAGGTCGTATTTGGTATTTGG	20	24	50.0	41.7	58.0	57.6	0.0	0.0	4.397421	1371	1994	686-723	1.000000	54.5
matK-trnQ-UUG_p1	matK-trnQ-UUG	1	GCATCTTTCACCCAACAACG	GGCGTAGTCAAGTGGTAAGG	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.056448	3540	3951	994-1008	1.000000	85.5
matK-trnQ-UUG_p2	matK-trnQ-UUG	2	GCATCTTTCACCCAACAACG	GGGCGTAGTCAAGTGGTAAG	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.056448	3540	3951	995-1009	1.000000	85.5
matK-trnQ-UUG_p3	matK-trnQ-UUG	3	CCGAGTTCGCTCTAGGTCTA	GGCGTAGTCAAGTGGTAAGG	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.055142	3540	3951	523-523	0.500000	59.6
matK-trnQ-UUG_p4	matK-trnQ-UUG	4	CCGAGTTCGCTCTAGGTCTA	GGGCGTAGTCAAGTGGTAAG	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.055142	3540	3951	524-524	0.500000	59.6
matK-trnQ-UUG_p5	matK-trnQ-UUG	5	GTTTCCGAGTTCGCTCTAGG	GGCGTAGTCAAGTGGTAAGG	20	20	55.0	55.0	58.1	58.0	0.0	0.0	0.095283	3540	3951	527-527	0.500000	59.2
atpI-rps2_p1	atpI-rps2	1	TGGTGGAACATAATAGAACATCCA	CGCAATTTGTGAAGGTCGTT	24	20	37.5	45.0	57.7	57.6	0.0	3.8	4.719830	10253	10471	287-349	1.000000	49.5
atpI-rps2_p2	atpI-rps2	2	TGGTGGAACATAATAGAACATCCA	TCGCAATTTGTGAAGGTCGT	24	20	37.5	45.0	57.7	58.4	0.0	10.1	4.731760	10253	10471	288-350	1.000000	49.4
atpI-rps2_p3	atpI-rps2	3	TGGTGGAACATAATAGAACATCCA	TTCGCAATTTGTGAAGGTCG	24	20	37.5	45.0	57.7	57.3	0.0	18.8	4.999788	10253	10471	289-351	1.000000	47.3
atpI-rps2_p4	atpI-rps2	4	GGTGGAACATAATAGAACATCCA	CGCAATTTGTGAAGGTCGTT	23	20	39.1	45.0	56.1	57.6	0.0	3.8	5.343342	10253	10471	286-348	1.000000	47.3
atpI-rps2_p5	atpI-rps2	5	TGGTGGAACATAATAGAACATCC	CGCAATTTGTGAAGGTCGTT	23	20	39.1	45.0	56.1	57.6	0.0	3.8	5.343342	10253	10471	287-349	1.000000	47.3
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	TCATTTCCATCCCAGAGCAT	CCGGATTTGAACTGGGGAAA	20	20	45.0	50.0	56.6	58.1	0.0	0.0	1.517545	21815	22657	717-925	1.000000	77.7
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	TCATTTCCATCCCAGAGCAT	AACTGGGGAAAGGGGATTTG	20	20	45.0	50.0	56.6	57.7	0.0	0.0	1.761078	21815	22657	708-916	1.000000	75.8
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	TCATTTCCATCCCAGAGCAT	ATTTGAACTGGGGAAAGGGG	20	20	45.0	50.0	56.6	57.7	0.0	0.0	1.761078	21815	22657	713-921	1.000000	75.8
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	TCATTTCCATCCCAGAGCAT	TGAACTGGGGAAAGGGGATT	20	20	45.0	50.0	56.6	58.5	0.0	0.0	1.968559	21815	22657	710-918	1.000000	74.1
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	TCATTTCCATCCCAGAGCAT	TTGAACTGGGGAAAGGGGAT	20	20	45.0	50.0	56.6	58.5	0.0	0.0	1.968559	21815	22657	711-919	1.000000	74.1
petN-psbM_p1	petN-psbM	1	ATCGCTTGGGCTGCTTTAAT	ACGTAAAAACGGCTAGTCAA	20	20	45.0	40.0	58.2	55.4	0.0	2.8	2.881970	23076	23566	562-604	1.000000	63.6
petN-psbM_p2	petN-psbM	2	ATCGCTTGGGCTGCTTTAAT	TCCTTTACGTAAAAACGGCT	20	20	45.0	40.0	58.2	55.3	0.0	20.5	2.913057	23076	23566	568-610	1.000000	63.3
petN-psbM_p3	petN-psbM	3	GTATCGCTTGGGCTGCTTTA	ACGTAAAAACGGCTAGTCAA	20	20	50.0	40.0	58.3	55.4	0.0	2.8	2.986034	23076	23566	564-606	1.000000	62.7
petN-psbM_p4	petN-psbM	4	GTATCGCTTGGGCTGCTTTA	TCCTTTACGTAAAAACGGCT	20	20	50.0	40.0	58.3	55.3	0.0	20.5	3.017121	23076	23566	570-612	1.000000	62.4
petN-psbM_p5	petN-psbM	5	TATCGCTTGGGCTGCTTTAA	ACGTAAAAACGGCTAGTCAA	20	20	45.0	40.0	57.6	55.4	0.0	2.8	3.061356	23076	23566	563-605	1.000000	62.1
psbM-trnD-GUC_p1	psbM-trnD-GUC	1	ATCGCTTGGGCTGCTTTAAT	GAACCGATGACTTACGCCTT	20	20	45.0	50.0	58.2	58.0	0.0	0.0	0.238212	23681	24304	1987-2536	1.000000	85.3
psbM-trnD-GUC_p2	psbM-trnD-GUC	2	ATCGCTTGGGCTGCTTTAAT	CCATGGCGTTACTCTACCAC	20	20	45.0	55.0	58.2	58.1	0.0	0.0	0.296309	23681	24304	1966-2515	1.000000	84.9
psbM-trnD-GUC_p3	psbM-trnD-GUC	3	ATCGCTTGGGCTGCTTTAAT	TGGCGTTACTCTACCACTGA	20	20	45.0	50.0	58.2	58.1	0.0	0.0	0.318463	23681	24304	1963-2512	1.000000	84.7
psbM-trnD-GUC_p4	psbM-trnD-GUC	4	GTATCGCTTGGGCTGCTTTA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	58.3	58.0	0.0	0.0	0.342276	23681	24304	1989-2538	1.000000	84.4
psbM-trnD-GUC_p5	psbM-trnD-GUC	5	ATCGCTTGGGCTGCTTTAAT	CCGATGACTTACGCCTTACC	20	20	45.0	55.0	58.2	58.2	0.0	0.0	0.383145	23681	24304	1984-2533	1.000000	84.1
trnY-GUA-trnE-UUC_p1	trnY-GUA-trnE-UUC	1	CCAGCGTAGACATATTGCCA	CTCTCTTTCAAGGAGGCAGC	20	20	50.0	55.0	57.8	58.3	0.0	0.0	0.502330	24541	24607	173-176	1.000000	79.6
trnY-GUA-trnE-UUC_p2	trnY-GUA-trnE-UUC	2	ACCAGCGTAGACATATTGCC	CTCTCTTTCAAGGAGGCAGC	20	20	50.0	55.0	57.8	58.3	0.0	0.0	0.503882	24541	24607	174-177	1.000000	79.6
trnY-GUA-trnE-UUC_p3	trnY-GUA-trnE-UUC	3	CCAGCGTAGACATATTGCCA	TCGTCTAGTGGTTCAGGACA	20	20	50.0	50.0	57.8	57.7	0.0	0.0	0.520481	24541	24607	194-197	1.000000	79.1
trnY-GUA-trnE-UUC_p4	trnY-GUA-trnE-UUC	4	ACCAGCGTAGACATATTGCC	TCGTCTAGTGGTTCAGGACA	20	20	50.0	50.0	57.8	57.7	0.0	0.0	0.522032	24541	24607	195-198	1.000000	79.1
trnY-GUA-trnE-UUC_p5	trnY-GUA-trnE-UUC	5	CAACGAATTTACAGTCCGCC	CTCTCTTTCAAGGAGGCAGC	20	20	50.0	55.0	57.5	58.3	0.0	0.0	0.784075	24541	24607	155-158	1.000000	77.8
ycf3-trnS-GGA_p1	ycf3-trnS-GGA	1	ATTTATCCGTGATCTAGGCA	TTCCAATGCTACGCCTTGAA	20	20	40.0	45.0	53.6	57.8	0.0	5.6	4.583930	37426	38095	722-729	1.000000	52.5
ycf3-trnS-GGA_p2	ycf3-trnS-GGA	2	TTTATCCGTGATCTAGGCAT	TTCCAATGCTACGCCTTGAA	20	20	40.0	45.0	53.6	57.8	0.0	5.6	4.583930	37426	38095	721-728	1.000000	52.5
ycf3-trnS-GGA_p3	ycf3-trnS-GGA	3	ATTTATCCGTGATCTAGGCA	ACAGTGATGGGTGAAGTGTG	20	20	40.0	50.0	53.6	57.7	0.0	0.0	4.652110	37426	38095	790-797	1.000000	51.5
ycf3-trnS-GGA_p4	ycf3-trnS-GGA	4	TTTATCCGTGATCTAGGCAT	ACAGTGATGGGTGAAGTGTG	20	20	40.0	50.0	53.6	57.7	0.0	0.0	4.652110	37426	38095	789-796	1.000000	51.5
ycf3-trnS-GGA_p5	ycf3-trnS-GGA	5	TTTATCCGTGATCTAGGCAT	GTGATGGGTGAAGTGTGGAA	20	20	40.0	50.0	53.6	57.7	0.0	0.0	4.661618	37426	38095	786-793	1.000000	51.5
trnS-GGA-rps4_p1	trnS-GGA-rps4	1	CACTCCGTTTTGGGTTCAGA	AGTAAATCGGTCGGTTTGCA	20	20	50.0	45.0	58.0	57.8	0.0	16.7	0.211058	38184	38330	332-350	1.000000	81.6
trnS-GGA-rps4_p2	trnS-GGA-rps4	2	TCACTCCGTTTTGGGTTCAG	AGTAAATCGGTCGGTTTGCA	20	20	50.0	45.0	58.0	57.8	0.0	16.7	0.211058	38184	38330	333-351	1.000000	81.6
trnS-GGA-rps4_p3	trnS-GGA-rps4	3	GGCTTTTTGTTTACCGAGGG	AGTAAATCGGTCGGTTTGCA	20	20	50.0	45.0	57.3	57.8	0.0	16.7	0.890431	38184	38330	253-271	1.000000	77.2
trnS-GGA-rps4_p4	trnS-GGA-rps4	4	TTACGTAGGAGAGATGGCCG	AGTAAATCGGTCGGTTTGCA	20	20	55.0	45.0	58.7	57.8	0.0	16.7	0.857161	38184	38330	308-326	1.000000	76.7
trnS-GGA-rps4_p5	trnS-GGA-rps4	5	ACTCCGTTTTGGGTTCAGAA	AGTAAATCGGTCGGTTTGCA	20	20	45.0	45.0	57.3	57.8	0.0	16.7	0.894276	38184	38330	331-349	1.000000	76.1
atpB-rbcL_p1	atpB-rbcL	1	ATTCGTCCGAGAGTTGCTTC	GTAAGTCCATCGGTCCACAC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.008301	42785	43550	1319-1349	1.000000	85.6
atpB-rbcL_p2	atpB-rbcL	2	TCATAGCTACGGTCCGAACT	GTAAGTCCATCGGTCCACAC	20	20	50.0	55.0	58.0	58.0	20.5	0.0	0.054415	42785	43550	1228-1258	1.000000	85.6
atpB-rbcL_p3	atpB-rbcL	3	ACTCATAGCTACGGTCCGAA	GTAAGTCCATCGGTCCACAC	20	20	50.0	55.0	58.0	58.0	20.5	0.0	0.054415	42785	43550	1230-1260	1.000000	85.5
atpB-rbcL_p4	atpB-rbcL	4	ATTCGTCCGAGAGTTGCTTC	CGATCAAGGCTGGTAAGTCC	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.061006	42785	43550	1331-1361	1.000000	85.1
atpB-rbcL_p5	atpB-rbcL	5	ATTCGTCCGAGAGTTGCTTC	CTCTCCAGCGCATAAATGGT	20	20	50.0	50.0	58.0	58.0	0.0	11.5	0.043289	42785	43550	1741-1771	1.000000	84.2
rpl16_p1	rpl16	1	GTTGCGTCTTTGATCACAGC	ATCGAGTGGATCAGAGAGGG	20	20	50.0	55.0	58.0	58.0	3.9	0.0	0.050282	66820	67617	1267-1661	1.000000	88.1
rpl16_p2	rpl16	2	GTTGCGTCTTTGATCACAGC	GTCGGATCGACGGAAAAGAA	20	20	50.0	50.0	58.0	58.0	3.9	4.7	0.057540	66820	67617	1296-1690	1.000000	87.9
rpl16_p3	rpl16	3	TCTGGATCAACGACAACTGC	ATCGAGTGGATCAGAGAGGG	20	20	50.0	55.0	58.2	58.0	0.0	0.0	0.210276	66820	67617	1396-1790	1.000000	86.2
rpl16_p4	rpl16	4	TCTGGATCAACGACAACTGC	GTCGGATCGACGGAAAAGAA	20	20	50.0	50.0	58.2	58.0	0.0	4.7	0.217535	66820	67617	1425-1819	1.000000	86.0
rpl16_p5	rpl16	5	GTTGCGTCTTTGATCACAGC	GTCGACGGTATTGCGTGTAT	20	20	50.0	50.0	58.0	58.2	3.9	0.0	0.212895	66820	67617	1624-2018	1.000000	85.5
ycf1_p1	ycf1	1	CTTTTGGTCCTCCCCGAAAA	ACGTTTCATGATAGTGAAGGACA	20	23	50.0	39.1	58.0	57.9	0.0	0.0	3.157623	93598	93717	783-783	0.500000	34.7
ycf1_p2	ycf1	2	TTTGGTCCTCCCCGAAAAAG	ACGTTTCATGATAGTGAAGGACA	20	23	50.0	39.1	58.0	57.9	0.0	0.0	3.157623	93598	93717	781-781	0.500000	34.7
ycf1_p3	ycf1	3	GGGAATTTCCGCAAAACTGG	ACGTTTCATGATAGTGAAGGACA	20	23	50.0	39.1	57.9	57.9	9.3	0.0	3.226178	93598	93717	969-969	0.500000	34.2
ycf1_p4	ycf1	4	GCTGAAATGCCTTTTGGTCC	ACGTTTCATGATAGTGAAGGACA	20	23	50.0	39.1	57.9	57.9	0.0	0.0	3.231939	93598	93717	793-793	0.500000	34.1
ycf1_p5	ycf1	5	ATCCAAAGGCGTAAAACGGT	ACGTTTCATGATAGTGAAGGACA	20	23	45.0	39.1	58.1	57.9	1.3	0.0	3.247084	93598	93717	1441-1441	0.500000	34.0
ycf1-psaC_p1	ycf1-psaC	1	TGAACCGATTCGTCTGTCTG	TACAACCGACACAGTCCTCT	20	20	50.0	50.0	57.7	58.0	5.2	0.0	0.356576	95892	96306	901-1020	1.000000	86.8
ycf1-psaC_p2	ycf1-psaC	2	ACCAAATCGATGAAAGGGGG	TACAACCGACACAGTCCTCT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.152755	95892	96306	1052-1052	0.500000	58.8
ycf1-psaC_p3	ycf1-psaC	3	ACCAAATCGATGAAAGGGGG	CAGTCCTCTGTTCTTGGAGC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.235728	95892	96306	1041-1041	0.500000	58.1
ycf1-psaC_p4	ycf1-psaC	4	ACCAAATCGATGAAAGGGGG	TTTGCTTAGCTTTGCAACCG	20	20	50.0	45.0	57.9	57.9	0.0	20.6	0.276616	95892	96306	1013-1013	0.500000	57.8
ycf1-psaC_p5	ycf1-psaC	5	ACCAAATCGATGAAAGGGGG	GGGAGCTGTATGGGCTAATG	20	20	50.0	55.0	57.9	57.8	0.0	0.0	0.335003	95892	96306	1584-1584	0.500000	57.3
psaC-ccsA_p1	psaC-ccsA	1	AGAGGACTGTGTCGGTTGTA	TCCAAAAGAAACGTGGGCAT	20	20	50.0	45.0	58.0	58.3	0.0	0.0	0.310373	96553	96742	504-800	1.000000	84.5
psaC-ccsA_p2	psaC-ccsA	2	AGAGGACTGTGTCGGTTGTA	GGGAGCTGTATGGGCTAATG	20	20	50.0	55.0	58.0	57.8	0.0	0.0	0.200007	96553	96742	552-552	0.500000	58.4
psaC-ccsA_p3	psaC-ccsA	3	CAAATTGCTTCCGCTCCAAG	GGGAGCTGTATGGGCTAATG	20	20	50.0	55.0	58.0	57.8	0.0	0.0	0.204430	96553	96742	575-575	0.500000	58.4
psaC-ccsA_p4	psaC-ccsA	4	AAATTGCTTCCGCTCCAAGA	GGGAGCTGTATGGGCTAATG	20	20	45.0	55.0	58.1	57.8	0.0	0.0	0.281428	96553	96742	574-574	0.500000	57.7
psaC-ccsA_p5	psaC-ccsA	5	AATTGCTTCCGCTCCAAGAA	GGGAGCTGTATGGGCTAATG	20	20	45.0	55.0	58.1	57.8	0.0	0.0	0.281428	96553	96742	573-573	0.500000	57.7
ccsA_p1	ccsA	1	AGAGGACTGTGTCGGTTGTA	ACGTTGCTCTTAGGAAGCAG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.094933	96743	97684	1409-1626	1.000000	88.4
ccsA_p2	ccsA	2	AGAGGACTGTGTCGGTTGTA	CGTTGCTCTTAGGAAGCAGT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.094933	96743	97684	1408-1625	1.000000	88.4
ccsA_p3	ccsA	3	AGAGGACTGTGTCGGTTGTA	GGGGCTCTGGACAAAATTGA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.085523	96743	97684	1532-1737	1.000000	88.3
ccsA_p4	ccsA	4	CAAATTGCTTCCGCTCCAAG	GGGGCTCTGGACAAAATTGA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.089946	96743	97684	1555-1760	1.000000	88.3
ccsA_p5	ccsA	5	CAAATTGCTTCCGCTCCAAG	CGTTGCTCTTAGGAAGCAGT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.099356	96743	97684	1431-1648	1.000000	88.3