Hotspot

rps15

Genome · 101971-102237 · 267 bp

Back to Pleurozia

Pi 0.015

MarkerSeek score 47.30

Primer available yes

Label rps15

Feature Metadata

label_name: rps15
feature_type: gene
region: Genome
length_bp: 267
variable_sites: 6
pi: 0.015
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15_p1	TACTGTTCGGTTTTCGGCAA	GAGCATCGTTTACCTCGGTT	45.0	50.0	58.0	58.0	1043-1116 bp	1.0	85.8
rps15_p2	TTACTGTTCGGTTTTCGGCA	GAGCATCGTTTACCTCGGTT	45.0	50.0	58.0	58.0	1044-1117 bp	1.0	85.8
rps15_p3	TGATGGATGATGAGGACCCA	GAGCATCGTTTACCTCGGTT	50.0	50.0	57.8	58.0	926-999 bp	1.0	84.9
rps15_p4	ATTCGCATACCAGTAGCAGC	GAGCATCGTTTACCTCGGTT	50.0	50.0	58.1	58.0	1321-1394 bp	1.0	84.8
rps15_p5	AGCCATAAAAGGACCAAGCC	GAGCATCGTTTACCTCGGTT	50.0	50.0	58.1	58.0	1232-1305 bp	1.0	84.7