Hotspot

psbZ

Genome · 41166-41354 · 189 bp

Back to Pleurozia

Pi 0.00705

MarkerSeek score 43.00

Primer available yes

Label psbZ

Feature Metadata

label_name: psbZ
feature_type: gene
region: Genome
length_bp: 189
variable_sites: 2
pi: 0.00705
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ_p1	GATCTTCGTGCTCCATGGTT	CGGAGTAGAGCAGTCTGGTA	50.0	55.0	58.0	58.0	1248-1263 bp	1.0	83.1
psbZ_p2	GATCTTCGTGCTCCATGGTT	AGTCGTCTTCATCGTCGTTG	50.0	50.0	58.0	58.0	1474-1490 bp	1.0	82.8
psbZ_p3	GATCTTCGTGCTCCATGGTT	CAAGTCGTCTTCATCGTCGT	50.0	50.0	58.0	58.0	1476-1492 bp	1.0	82.8
psbZ_p4	CGTTTTTGGGATCTTCGTGC	AGTCGTCTTCATCGTCGTTG	50.0	50.0	58.0	58.0	1483-1499 bp	1.0	82.8
psbZ_p5	CGTTTTTGGGATCTTCGTGC	CAAGTCGTCTTCATCGTCGT	50.0	50.0	58.0	58.0	1485-1501 bp	1.0	82.8