Hotspot

rpl32-trnL-UAG

Genome · 92646-93859 · 1214 bp

Back to Pleurozia

Pi 0.0318
MarkerSeek score 68.80
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
Genome
length_bp
1214
variable_sites
57
pi
0.0318
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
68.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TCATCTGAATCCGTATCAACTGA TTCGAACCGAGATGCGTTAG 39.1 50.0 57.1 58.1 1287-1313 bp 1.0 53.7
rpl32-trnL-UAG_p2 TCTGAATCCGTATCAACTGATGA TTCGAACCGAGATGCGTTAG 39.1 50.0 57.1 58.1 1284-1310 bp 1.0 53.7
rpl32-trnL-UAG_p3 TCTGAATCCGTATCAACTGATGA GCTTCCTAAGAGCAACGTGT 39.1 50.0 57.1 58.2 1260-1286 bp 1.0 53.0
rpl32-trnL-UAG_p4 TCATCTGAATCCGTATCAACTGA GCTTCCTAAGAGCAACGTGT 39.1 50.0 57.1 58.2 1263-1289 bp 1.0 52.9
rpl32-trnL-UAG_p5 TCTGAATCCGTATCAACTGATGA TGCTTCCTAAGAGCAACGTG 39.1 50.0 57.1 58.2 1261-1287 bp 1.0 52.9