Hotspot

ndhF-rpl21

Genome · 91613-91959 · 347 bp

Back to Pleurozia

Pi 0.0374

MarkerSeek score 66.80

Primer available yes

Label ndhF-rpl21

Feature Metadata

label_name: ndhF-rpl21
feature_type: igs
region: Genome
length_bp: 347
variable_sites: 19
pi: 0.0374
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl21_p1	CCGCACTAGGTCTGGTAAAC	CAGGTTCTACTCGGAGTTGC	55.0	55.0	58.0	58.0	913-917 bp	1.0	84.1
ndhF-rpl21_p2	CCGCACTAGGTCTGGTAAAC	GGTTCTACTCGGAGTTGCTG	55.0	55.0	58.0	58.0	911-915 bp	1.0	84.1
ndhF-rpl21_p3	CGCACTAGGTCTGGTAAACC	CAGGTTCTACTCGGAGTTGC	55.0	55.0	58.0	58.0	912-916 bp	1.0	84.1
ndhF-rpl21_p4	CGCACTAGGTCTGGTAAACC	GGTTCTACTCGGAGTTGCTG	55.0	55.0	58.0	58.0	910-914 bp	1.0	84.1
ndhF-rpl21_p5	CGCACTAGGTCTGGTAAACC	ACCCTTAACTATGGCACCCT	55.0	50.0	58.0	58.0	1064-1068 bp	1.0	83.8