Hotspot

trnM-CAU-trnV-GAC

Genome · 80788-81402 · 615 bp

Back to Pleurozia

Pi 0.0217

MarkerSeek score 61.70

Primer available yes

Label trnM-CAU-trnV-GAC

Feature Metadata

label_name: trnM-CAU-trnV-GAC
feature_type: igs
region: Genome
length_bp: 615
variable_sites: 17
pi: 0.0217
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-trnV-GAC_p1	AACCTGTGAGTTCGCCAATT	TCTACCACTGAGTTATACCCCT	45.0	45.5	58.0	57.1	485-700 bp	1.0	62.9
trnM-CAU-trnV-GAC_p2	AACCTGTGAGTTCGCCAATT	CTCTACCACTGAGTTATACCCCT	45.0	47.8	58.0	58.2	486-701 bp	1.0	60.5
trnM-CAU-trnV-GAC_p3	AACCTGTGAGTTCGCCAATT	CTCTACCACTGAGTTATACCCC	45.0	50.0	58.0	56.8	486-701 bp	1.0	60.4
trnM-CAU-trnV-GAC_p4	GAACCTGTGAGTTCGCCAAT	TCTACCACTGAGTTATACCCCT	50.0	45.5	58.5	57.1	486-701 bp	1.0	59.2
trnM-CAU-trnV-GAC_p5	AACCTGTGAGTTCGCCAATT	TCTACCACTGAGTTATACCCC	45.0	47.6	58.0	55.6	485-700 bp	1.0	58.7