Hotspot

rpoA-rps11

Genome · 74372-74394 · 23 bp

Back to Pleurozia

Pi 0.087

MarkerSeek score 77.80

Primer available yes

Label rpoA-rps11

Feature Metadata

label_name: rpoA-rps11
feature_type: igs
region: Genome
length_bp: 23
variable_sites: 3
pi: 0.087
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA-rps11_p1	GGGCCTTTGATATCTTGAGCA	GGCTTCGAGCAAAATCTTCG	47.6	50.0	58.1	57.8	1004-1005 bp	0.666667	58.4
rpoA-rps11_p2	TGGGCCTTTGATATCTTGAGC	GGCTTCGAGCAAAATCTTCG	47.6	50.0	58.1	57.8	1005-1006 bp	0.666667	58.4
rpoA-rps11_p3	GGGCCTTTGATATCTTGAGCA	ACAGATATACGGGGGCAAGT	47.6	50.0	58.1	58.2	679-682 bp	0.666667	58.2
rpoA-rps11_p4	TGGGCCTTTGATATCTTGAGC	ACAGATATACGGGGGCAAGT	47.6	50.0	58.1	58.2	680-683 bp	0.666667	58.2
rpoA-rps11_p5	AGTCAGACTTCCATCAGTCCA	GGCTTCGAGCAAAATCTTCG	47.6	50.0	58.1	57.8	1263-1264 bp	0.666667	58.1