Hotspot

petB-petD

Genome · 72110-72219 · 110 bp

Back to Pleurozia

Pi 0.071

MarkerSeek score 77.60

Primer available yes

Label petB-petD

Feature Metadata

label_name: petB-petD
feature_type: igs
region: Genome
length_bp: 110
variable_sites: 11
pi: 0.071
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 77.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB-petD_p1	AAACAAGGGATTTCGGGTCC	AAGATCATTAGGCCAAGCGG	50.0	50.0	58.1	58.0	799-804 bp	1.0	82.3
petB-petD_p2	AAAAACCCCGCGAGTTAACT	AAGATCATTAGGCCAAGCGG	45.0	50.0	58.0	58.0	1089-1094 bp	1.0	82.2
petB-petD_p3	AAAACCCCGCGAGTTAACTT	AAGATCATTAGGCCAAGCGG	45.0	50.0	58.0	58.0	1088-1093 bp	1.0	82.2
petB-petD_p4	CCGCGAGTTAACTTGGGTAA	AAGATCATTAGGCCAAGCGG	50.0	50.0	57.9	58.0	1082-1087 bp	1.0	81.8
petB-petD_p5	TGATCCGTTTGAGCCGTATG	AAGATCATTAGGCCAAGCGG	50.0	50.0	58.1	58.0	1486-1491 bp	1.0	81.4