Hotspot

petG-trnW-CCA

Genome · 64064-64148 · 85 bp

Back to Pleurozia

Pi 0.051

MarkerSeek score 78.40

Primer available yes

Label petG-trnW-CCA

Feature Metadata

label_name: petG-trnW-CCA
feature_type: igs
region: Genome
length_bp: 85
variable_sites: 6
pi: 0.051
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petG-trnW-CCA_p1	TACAATACCGACGTGGGGAT	CTCCAAAACCTGATGCCGTA	50.0	50.0	58.2	57.9	162-163 bp	1.0	80.3
petG-trnW-CCA_p2	TACAATACCGACGTGGGGAT	TCCAAAACCTGATGCCGTAG	50.0	50.0	58.2	57.9	161-162 bp	1.0	80.3
petG-trnW-CCA_p3	TACAATACCGACGTGGGGAT	CAGTTTGGTAGAACGCAGGT	50.0	50.0	58.2	58.1	182-183 bp	1.0	79.9
petG-trnW-CCA_p4	TACAATACCGACGTGGGGAT	GTTTGGTAGAACGCAGGTCT	50.0	50.0	58.2	57.8	180-181 bp	1.0	79.6
petG-trnW-CCA_p5	TACAATACCGACGTGGGGAT	TCAGTTTGGTAGAACGCAGG	50.0	50.0	58.2	57.8	183-184 bp	1.0	79.5