Hotspot

psbA-trnH-GUG

Genome · 29694-29846 · 153 bp

Back to Pleurozia

Pi 0.0697

MarkerSeek score 79.30

Primer available yes

Label psbA-trnH-GUG

Feature Metadata

label_name: psbA-trnH-GUG
feature_type: igs
region: Genome
length_bp: 153
variable_sites: 14
pi: 0.0697
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnH-GUG_p1	GCTCCAGCTGTTAACGGTTA	CTGCCTTAATCCACTTGGCT	50.0	50.0	57.9	57.9	199-213 bp	1.0	84.9
psbA-trnH-GUG_p2	GCTCCAGCTGTTAACGGTTA	ACTGCCTTAATCCACTTGGC	50.0	50.0	57.9	58.2	200-214 bp	1.0	84.6
psbA-trnH-GUG_p3	GCTCCAGCTGTTAACGGTTA	GATCCACAATCCACTGCCTT	50.0	50.0	57.9	57.9	212-226 bp	1.0	84.5
psbA-trnH-GUG_p4	TGAACGTAACGCTCACAACT	CTGCCTTAATCCACTTGGCT	45.0	50.0	57.8	57.9	245-259 bp	1.0	82.6
psbA-trnH-GUG_p5	TGAACGTAACGCTCACAACT	GATCCACAATCCACTGCCTT	45.0	50.0	57.8	57.9	258-272 bp	1.0	82.4