Hotspot

ycf1

SSC · 126310-131676 · 5367 bp

Back to Pleurothallis

Pi 0.0186

MarkerSeek score 48.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5367
variable_sites: 100
pi: 0.0186
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGCCTTATGTTTTCTCCAAGGT	GGAATGGAATAGAGAAATGCA	40.9	38.1	57.9	53.6	169 bp	1.0	44.3
ycf1_p2	TGCCTTATGTTTTCTCCAAGGT	AGGAATGGAATAGAGAAATGCA	40.9	36.4	57.9	55.2	170 bp	1.0	44.2
ycf1_p3	TGCCTTATGTTTTCTCCAAGGT	AAGGAATGGAATAGAGAAATGCA	40.9	34.8	57.9	56.0	171 bp	1.0	44.2
ycf1_p4	GTGCCTTATGTTTTCTCCAAGG	AGGAATGGAATAGAGAAATGCA	45.5	36.4	57.6	55.2	171 bp	1.0	44.2
ycf1_p5	TGCCTTATGTTTTCTCCAAGGT	AGGAATGGAATAGAGAAATGCAT	40.9	34.8	57.9	55.7	170 bp	1.0	44.2