Hotspot

rpl16

LSC · 82450-84093 · 1644 bp

Back to Pleurothallis

Pi 0.0251

MarkerSeek score 62.00

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1644
variable_sites: 40
pi: 0.0251
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	GCTCCACTGTTATCCGCTAC	GCAGGGTCCCTTTACAAACA	55.0	50.0	58.1	58.0	2072-2123 bp	1.0	83.2
rpl16_p2	TAACCGCTGCATTGTCATCA	GCAGGGTCCCTTTACAAACA	45.0	50.0	57.9	58.0	2281-2332 bp	1.0	83.1
rpl16_p3	TGATAACCGCTGCATTGTCA	GCAGGGTCCCTTTACAAACA	45.0	50.0	57.9	58.0	2284-2335 bp	1.0	83.1
rpl16_p4	TTTCTAGAGGCATGTTGGGC	GCAGGGTCCCTTTACAAACA	50.0	50.0	57.9	58.0	2185-2236 bp	1.0	83.1
rpl16_p5	TAACCGCTGCATTGTCATCA	TCTCGCCATTATGCTCATCG	45.0	50.0	57.9	57.9	2134-2185 bp	1.0	82.7