Hotspot

clpP1

LSC · 70099-72291 · 2193 bp

Back to Pleurothallis

Pi 0.0147

MarkerSeek score 53.50

Primer available yes

Label clpP1

Feature Metadata

label_name: clpP1
feature_type: gene
region: LSC
length_bp: 2193
variable_sites: 32
pi: 0.0147
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 53.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP1_p1	GCGGGTTGGTTTAGATCGAT	GATCGATCCCGTTCGTTACC	50.0	55.0	58.1	58.2	2755 bp	0.5	57.7
clpP1_p2	TAATGGTTGGCATGGCATGT	GATCGATCCCGTTCGTTACC	45.0	55.0	58.1	58.2	2804 bp	0.5	57.0
clpP1_p3	AGTCGCACATACACCCTAGT	GATCGATCCCGTTCGTTACC	50.0	55.0	58.2	58.2	2913 bp	0.5	56.9
clpP1_p4	TGGCTGTCTTGCGTTTCTAA	GATCGATCCCGTTCGTTACC	45.0	55.0	57.8	58.2	2833 bp	0.5	56.1
clpP1_p5	TTGGCTGTCTTGCGTTTCTA	GATCGATCCCGTTCGTTACC	45.0	55.0	57.8	58.2	2834 bp	0.5	56.1