Hotspot

atpF

LSC · 11568-13062 · 1495 bp

Back to Pleurothallis

Pi 0.0228

MarkerSeek score 58.70

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1495
variable_sites: 34
pi: 0.0228
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	AGAGTAAAAAGGCTAATCAGT	CTATCTATAAGGGGAGAGCA	33.3	45.0	52.2	52.4	1540-1541 bp	1.0	42.0
atpF_p2	AGAGTAAAAAGGCTAATCAGTT	CTATCTATAAGGGGAGAGCA	31.8	45.0	53.1	52.4	1540-1541 bp	1.0	42.0
atpF_p3	AGAGTAAAAAGGCTAATCAGT	CTATCTATAAGGGGAGAGCAT	33.3	42.9	52.2	53.1	1540-1541 bp	1.0	42.0
atpF_p4	AGAGTAAAAAGGCTAATCAGTT	CTATCTATAAGGGGAGAGCAT	31.8	42.9	53.1	53.1	1540-1541 bp	1.0	42.0
atpF_p5	TAGAGTAAAAAGGCTAATCAGT	CTATCTATAAGGGGAGAGCA	31.8	45.0	52.3	52.4	1541-1542 bp	1.0	42.0