Hotspot

psaC-ndhE

SSC · 119813-120355 · 543 bp

Back to Pleurothallis

Pi 0.0231

MarkerSeek score 75.00

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 543
variable_sites: 12
pi: 0.0231
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 75.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	AGCTCGAACACATTGGGTAC	CTGAAGCAGCTATTGGGCTA	50.0	50.0	57.9	57.4	671-695 bp	1.0	78.4
psaC-ndhE_p2	AGCTCGAACACATTGGGTAC	TGAAGCAGCTATTGGGCTAG	50.0	50.0	57.9	57.4	670-694 bp	1.0	78.4
psaC-ndhE_p3	ACATCCGTCCCAAGGTATCA	TGAAGCAGCTATTGGGCTAG	50.0	50.0	58.1	57.4	712-736 bp	1.0	78.0
psaC-ndhE_p4	ACATCCGTCCCAAGGTATCA	CTGAAGCAGCTATTGGGCTA	50.0	50.0	58.1	57.4	713-737 bp	1.0	77.9
psaC-ndhE_p5	GCTCGAACACATTGGGTACA	TGAAGCAGCTATTGGGCTAG	50.0	50.0	58.2	57.4	669-693 bp	1.0	77.6