Hotspot

rpl16-rps3

LSC · 84094-84280 · 187 bp

Back to Pleurothallis

Pi 0.0608

MarkerSeek score 71.10

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 187
variable_sites: 11
pi: 0.0608
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 71.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	TCTGCAATGGAGCTCTCAAC	GCAGGGTCCCTTTACAAACA	50.0	50.0	57.9	58.0	978-1002 bp	1.0	83.4
rpl16-rps3_p2	TGAACTCTGCAATGGAGCTC	GCAGGGTCCCTTTACAAACA	50.0	50.0	57.9	58.0	983-1007 bp	1.0	83.3
rpl16-rps3_p3	CTGAACTCTGCAATGGAGCT	GCAGGGTCCCTTTACAAACA	50.0	50.0	57.9	58.0	984-1008 bp	1.0	83.3
rpl16-rps3_p4	TCTGCAATGGAGCTCTCAAC	GGACGAACACGAGGAATTGA	50.0	50.0	57.9	57.9	1288 bp	0.5	58.6
rpl16-rps3_p5	TGAACTCTGCAATGGAGCTC	GGACGAACACGAGGAATTGA	50.0	50.0	57.9	57.9	1293 bp	0.5	58.6