Hotspot

psaJ-rpl33

LSC · 67158-67768 · 611 bp

Back to Pleurothallis

Pi 0.0459

MarkerSeek score 79.10

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 611
variable_sites: 28
pi: 0.0459
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	GATGCCTTGTCATTCCCCTT	GCCTGGCGATTCTTTATTGC	50.0	50.0	57.9	57.9	731-747 bp	1.0	80.9
psaJ-rpl33_p2	GATGCCTTGTCATTCCCCTT	AGAAATGCCTGGCGATTCTT	50.0	45.0	57.9	57.9	737-753 bp	1.0	80.8
psaJ-rpl33_p3	TGGTTTGGGTCTTTAGCAGG	GCCTGGCGATTCTTTATTGC	50.0	50.0	57.7	57.9	782-798 bp	1.0	79.6
psaJ-rpl33_p4	GGTTTGGGTCTTTAGCAGGT	GCCTGGCGATTCTTTATTGC	50.0	50.0	57.7	57.9	781-797 bp	1.0	79.6
psaJ-rpl33_p5	TGGTTTGGGTCTTTAGCAGG	AGAAATGCCTGGCGATTCTT	50.0	45.0	57.7	57.9	788-804 bp	1.0	79.5