Hotspot

rbcL-accD

LSC · 55960-56828 · 869 bp

Back to Pleurothallis

Pi 0.0444
MarkerSeek score 73.70
Primer available yes
Label rbcL-accD

Feature Metadata

label_name
rbcL-accD
feature_type
igs
region
LSC
length_bp
869
variable_sites
37
pi
0.0444
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-accD_p1 AATGAGGGACGTGATCTTGC TTCTTCACTCCCACCGGTAT 50.0 50.0 58.0 58.1 1120-1145 bp 1.0 84.9
rbcL-accD_p2 ACTACAGTTCGGTGGAGGAA TTCTTCACTCCCACCGGTAT 50.0 50.0 58.0 58.1 1217-1242 bp 1.0 84.7
rbcL-accD_p3 GGCTTCAGGGGGTATTCATG TTCTTCACTCCCACCGGTAT 55.0 50.0 58.0 58.1 1283-1308 bp 1.0 84.3
rbcL-accD_p4 GAGCGGTATTTGCCAGAGAA TTCTTCACTCCCACCGGTAT 50.0 50.0 58.0 58.1 1656-1681 bp 1.0 83.2
rbcL-accD_p5 AAGAGCGGTATTTGCCAGAG TTCTTCACTCCCACCGGTAT 50.0 50.0 58.0 58.1 1658-1683 bp 1.0 83.2