Hotspot

psbC-trnS-UGA

LSC · 35206-35346 · 141 bp

Back to Pleurothallis

Pi 0.05

MarkerSeek score 81.90

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 141
variable_sites: 7
pi: 0.05
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	CCTGTTCTTTCCATGACCCC	GAGAGATGGCTGAGTGGTTG	55.0	55.0	58.2	58.0	263-264 bp	1.0	81.0
psbC-trnS-UGA_p2	GTTTGTGGGACATTTGTGGC	GAGAGATGGCTGAGTGGTTG	50.0	55.0	58.1	58.0	345-346 bp	1.0	80.6
psbC-trnS-UGA_p3	CCTGTTCTTTCCATGACCCC	ATAGCCCCGGTCTTGAAAAC	55.0	50.0	58.2	57.9	243-244 bp	1.0	80.3
psbC-trnS-UGA_p4	AGCGGGGTTTGAAAAAGGAA	GAGAGATGGCTGAGTGGTTG	45.0	55.0	58.2	58.0	300-301 bp	1.0	80.2
psbC-trnS-UGA_p5	GTTTGTGGGACATTTGTGGC	ATAGCCCCGGTCTTGAAAAC	50.0	50.0	58.1	57.9	325-326 bp	1.0	79.9