Hotspot

rpoB-trnC-GCA

LSC · 26515-27917 · 1403 bp

Back to Pleurothallis

Pi 0.0211

MarkerSeek score 75.40

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1403
variable_sites: 28
pi: 0.0211
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 75.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	AATTCTTCCGTCAAGCCCTG	TAAAGGATTTGCAGTCCCCC	50.0	50.0	58.2	57.5	1480-1551 bp	1.0	79.9
rpoB-trnC-GCA_p2	AATTCTTCCGTCAAGCCCTG	CGACACCCAGATTCGAACTG	50.0	55.0	58.2	58.6	1504-1575 bp	1.0	78.9
rpoB-trnC-GCA_p3	AATTCTTCCGTCAAGCCCTG	GTTGATCAGATCAGGCGACA	50.0	50.0	58.2	57.7	1590 bp	0.5	56.2
rpoB-trnC-GCA_p4	AATTCTTCCGTCAAGCCCTG	TGTTGATCAGATCAGGCGAC	50.0	50.0	58.2	57.7	1591 bp	0.5	56.2
rpoB-trnC-GCA_p5	AATTCTTCCGTCAAGCCCTG	TTGATCAGATCAGGCGACAC	50.0	50.0	58.2	57.7	1589 bp	0.5	56.2