Hotspot

psbI-trnS-GCU

LSC · 7686-7814 · 129 bp

Back to Pleurothallis

Pi 0.0775

MarkerSeek score 76.80

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 129
variable_sites: 10
pi: 0.0775
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	ATCTAATGATCCGGGGCGTA	TGGACTAAAGCGTCGGATTG	50.0	50.0	58.1	58.0	242 bp	1.0	82.7
psbI-trnS-GCU_p2	TATCTAATGATCCGGGGCGT	TGGACTAAAGCGTCGGATTG	50.0	50.0	58.1	58.0	243 bp	1.0	82.7
psbI-trnS-GCU_p3	ATCTAATGATCCGGGGCGTA	GTGGACTAAAGCGTCGGATT	50.0	50.0	58.1	58.0	243 bp	1.0	82.7
psbI-trnS-GCU_p4	TATCTAATGATCCGGGGCGT	GTGGACTAAAGCGTCGGATT	50.0	50.0	58.1	58.0	244 bp	1.0	82.7
psbI-trnS-GCU_p5	ATCTAATGATCCGGGGCGTA	GGGTTCGAATCCCTCTCTCT	50.0	55.0	58.1	58.2	192 bp	1.0	81.9