Hotspot

ndhF-rpl32

SSC · 114180-115368 · 1189 bp

Back to Pleurospermum

Pi 0.0206

MarkerSeek score 65.20

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1189
variable_sites: 72
pi: 0.0206
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GTTGCCGACGGAAAAAGAAG	TGAGCCGGTTTTTCGATTCA	50.0	45.0	58.0	58.1	1477-1519 bp	1.0	85.3
ndhF-rpl32_p2	TGCCGACGGAAAAAGAAGAA	TGAGCCGGTTTTTCGATTCA	45.0	45.0	58.0	58.1	1475-1517 bp	1.0	85.2
ndhF-rpl32_p3	TTGCCGACGGAAAAAGAAGA	TGAGCCGGTTTTTCGATTCA	45.0	45.0	58.0	58.1	1476-1518 bp	1.0	85.2
ndhF-rpl32_p4	CCCCAACGCGATTAGTTACA	TGAGCCGGTTTTTCGATTCA	50.0	45.0	58.0	58.1	1935-1977 bp	1.0	84.2
ndhF-rpl32_p5	CCGTGTGAACCAAAACCCTA	TGAGCCGGTTTTTCGATTCA	50.0	45.0	58.0	58.1	1883-1925 bp	1.0	84.2