Hotspot

rps19-rpl2

IRb · 85747-85804 · 58 bp

Back to Pleurospermum

Pi 0.0365

MarkerSeek score 67.30

Primer available yes

Label rps19-rpl2

Feature Metadata

label_name: rps19-rpl2
feature_type: igs
region: IRb
length_bp: 58
variable_sites: 5
pi: 0.0365
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-rpl2_p1	AACGATTTGCTACAAAAGGA	TTCTTCGTCGCCGTAGTAAA	35.0	45.0	53.4	57.0	118-132 bp	1.0	47.1
rps19-rpl2_p2	AACGATTTGCTACAAAAGGA	GATTCTTCGTCGCCGTAGTA	35.0	50.0	53.4	57.3	120-134 bp	1.0	47.0
rps19-rpl2_p3	ACGATTTGCTACAAAAGGAT	GATTCTTCGTCGCCGTAGTA	35.0	50.0	53.2	57.3	119-133 bp	1.0	47.0
rps19-rpl2_p4	AACGATTTGCTACAAAAGGA	GATTCTTCGTCGCCGTAGTAA	35.0	47.6	53.4	57.9	120-134 bp	1.0	47.0
rps19-rpl2_p5	AAACGATTTGCTACAAAAGGA	GATTCTTCGTCGCCGTAGTA	33.3	50.0	54.3	57.3	121-135 bp	1.0	46.9