Hotspot

psaJ-rpl33

LSC · 68646-69103 · 458 bp

Back to Pleurospermum

Pi 0.0249

MarkerSeek score 67.10

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 458
variable_sites: 30
pi: 0.0249
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TTTTCCCAGATGCGTTGACA	CACCGTTTCGAACACAACTG	45.0	50.0	58.0	57.9	548-561 bp	0.875	80.6
psaJ-rpl33_p2	ACATATCTTTCCGTGGCACC	CACCGTTTCGAACACAACTG	50.0	50.0	58.0	57.9	627-640 bp	0.875	79.7
psaJ-rpl33_p3	TAGTTTTCGGGAATGCTCGG	CACCGTTTCGAACACAACTG	50.0	50.0	58.0	57.9	842-855 bp	0.875	79.1
psaJ-rpl33_p4	CTGCGCTACATCCCTTCAAT	CACCGTTTCGAACACAACTG	50.0	50.0	58.0	57.9	1042-1055 bp	0.875	78.2
psaJ-rpl33_p5	CGAACTGAACTAAGAGCGCT	CACCGTTTCGAACACAACTG	50.0	50.0	58.0	57.9	1255-1273 bp	0.875	77.9