Hotspot

pafI-trnS-GGA

LSC · 45816-46688 · 873 bp

Back to Pleurospermum

Pi 0.0198

MarkerSeek score 66.40

Primer available yes

Label pafI-trnS-GGA

Feature Metadata

label_name: pafI-trnS-GGA
feature_type: igs
region: LSC
length_bp: 873
variable_sites: 52
pi: 0.0198
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
pafI-trnS-GGA_p1	CCCGATCCCAAATCATGTGT	TATTCGCGCCAGACCTAAAC	50.0	50.0	57.9	58.1	1389-1463 bp	1.0	86.8
pafI-trnS-GGA_p2	TGTTTGTTGTCCCGACCATT	TATTCGCGCCAGACCTAAAC	45.0	50.0	57.9	58.1	1703-1775 bp	1.0	86.5
pafI-trnS-GGA_p3	TGGCCTATTGTACTGGTGGA	CCCTCGGTAAACAAAAGCCT	50.0	50.0	58.0	58.1	1323-1336 bp	0.625	67.0
pafI-trnS-GGA_p4	TGGCCTATTGTACTGGTGGA	TATTCGCGCCAGACCTAAAC	50.0	50.0	58.0	58.1	1656-1664 bp	0.625	66.8
pafI-trnS-GGA_p5	TGGCCTATTGTACTGGTGGA	TGGGTATGGCTCCGACTATT	50.0	50.0	58.0	57.9	1952-1960 bp	0.625	66.3