Hotspot

psaA-pafI

LSC · 43131-43808 · 678 bp

Back to Pleurospermum

Pi 0.00953

MarkerSeek score 67.30

Primer available yes

Label psaA-pafI

Feature Metadata

label_name: psaA-pafI
feature_type: igs
region: LSC
length_bp: 678
variable_sites: 19
pi: 0.00953
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-pafI_p1	GCCCATTCCTCGAAAGAAGT	ATCAAGCCGCTGAGTATTGG	50.0	50.0	57.9	58.0	852-862 bp	1.0	80.9
psaA-pafI_p2	GCCCATTCCTCGAAAGAAGT	CGATCAAGCCGCTGAGTATT	50.0	50.0	57.9	58.1	854-864 bp	1.0	80.1
psaA-pafI_p3	GCCCATTCCTCGAAAGAAGT	TTGAAGATCACGAGGCGTTT	50.0	45.0	57.9	57.8	781-791 bp	1.0	80.1
psaA-pafI_p4	GCCCATTCCTCGAAAGAAGT	TTGGTTGAAGATCACGAGGC	50.0	50.0	57.9	58.2	785-795 bp	1.0	79.9
psaA-pafI_p5	GCCCATTCCTCGAAAGAAGT	AGCCGCTGAGTATTGGAAAC	50.0	50.0	57.9	58.3	848-858 bp	1.0	79.1