Hotspot

trnT-GGU-psbD

LSC · 32705-34134 · 1430 bp

Back to Pleurospermum

Pi 0.0242

MarkerSeek score 69.20

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1430
variable_sites: 97
pi: 0.0242
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	AATTGAATCAAACGGGCCCT	AAACTAGGCGCAAAGAACCA	45.0	45.0	58.1	58.0	2004-2066 bp	1.0	86.0
trnT-GGU-psbD_p2	AATTGAATCAAACGGGCCCT	AACTAGGCGCAAAGAACCAA	45.0	45.0	58.1	58.0	2003-2065 bp	1.0	86.0
trnT-GGU-psbD_p3	ATTGAATCAAACGGGCCCTT	AAACTAGGCGCAAAGAACCA	45.0	45.0	58.1	58.0	2003-2065 bp	1.0	86.0
trnT-GGU-psbD_p4	ATTGAATCAAACGGGCCCTT	AACTAGGCGCAAAGAACCAA	45.0	45.0	58.1	58.0	2002-2064 bp	1.0	86.0
trnT-GGU-psbD_p5	ATTGAATCAAACGGGCCCTT	ACCAAAACTAGGCGCAAAGA	45.0	45.0	58.1	58.0	2007-2069 bp	1.0	86.0