Hotspot

psbM-trnD-GUC

LSC · 30728-31418 · 691 bp

Back to Pleurospermum

Pi 0.019
MarkerSeek score 65.40
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
691
variable_sites
37
pi
0.019
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 AGCAGAAAAGCAGTAGGAACT TCCCCGGGATTGTAGTTCAA 42.9 50.0 57.2 58.3 837-853 bp 1.0 66.7
psbM-trnD-GUC_p2 AGCAGAAAAGCAGTAGGAACT TTCCCCGGGATTGTAGTTCA 42.9 50.0 57.2 58.3 838-854 bp 1.0 66.6
psbM-trnD-GUC_p3 AGCAGAAAAGCAGTAGGAACT TTCAATTGGTCAGAGCACCG 42.9 50.0 57.2 58.5 822-838 bp 1.0 65.6
psbM-trnD-GUC_p4 AAGCAGAAAAGCAGTAGGAACT TCCCCGGGATTGTAGTTCAA 40.9 50.0 57.9 58.3 838-854 bp 1.0 64.1
psbM-trnD-GUC_p5 AAGCAGAAAAGCAGTAGGAACT TTCCCCGGGATTGTAGTTCA 40.9 50.0 57.9 58.3 839-855 bp 1.0 64.1