Hotspot

trnG-UCC-trnR-UCU

LSC · 10605-10830 · 226 bp

Back to Pleurospermum

Pi 0.0303

MarkerSeek score 69.20

Primer available yes

Label trnG-UCC-trnR-UCU

Feature Metadata

label_name: trnG-UCC-trnR-UCU
feature_type: igs
region: LSC
length_bp: 226
variable_sites: 19
pi: 0.0303
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnR-UCU_p1	AGCCTTCCAAGCTAACGATG	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.0	56.9	276-306 bp	1.0	72.5
trnG-UCC-trnR-UCU_p2	AGCCTTCCAAGCTAACGATG	AGGTTTAGAAGACCTCTGTCCT	50.0	45.5	58.0	57.9	282-312 bp	1.0	71.7
trnG-UCC-trnR-UCU_p3	CTAACGATGCGGGTTCGATT	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.4	56.9	265-295 bp	1.0	69.5
trnG-UCC-trnR-UCU_p4	TAACGATGCGGGTTCGATTC	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.4	56.9	264-294 bp	1.0	69.5
trnG-UCC-trnR-UCU_p5	CTAACGATGCGGGTTCGATT	AGGTTTAGAAGACCTCTGTCCT	50.0	45.5	58.4	57.9	271-301 bp	1.0	68.7