Hotspot

trnS-GCU-trnG-UCC

LSC · 9092-9842 · 751 bp

Back to Pleurospermum

Pi 0.0274
MarkerSeek score 70.50
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
751
variable_sites
56
pi
0.0274
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.96
markerseek_score
70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 GCTTTAGTCCACTCAGCCAT GGGGGTTACTAGAACGAATCA 50.0 47.6 57.6 56.8 629-830 bp 1.0 69.2
trnS-GCU-trnG-UCC_p2 GCTTTAGTCCACTCAGCCAT GGGGGTTACTAGAACGAATCAC 50.0 50.0 57.6 58.2 629-830 bp 1.0 68.9
trnS-GCU-trnG-UCC_p3 AGTCCACTCAGCCATCTCTC GGGGGTTACTAGAACGAATCA 55.0 47.6 58.5 56.8 624-825 bp 1.0 68.4
trnS-GCU-trnG-UCC_p4 GCTTTAGTCCACTCAGCCAT AGGGGGTTACTAGAACGAATCA 50.0 45.5 57.6 58.3 630-831 bp 1.0 68.3
trnS-GCU-trnG-UCC_p5 AGTCCACTCAGCCATCTCTC GGGGGTTACTAGAACGAATCAC 55.0 50.0 58.5 58.2 624-825 bp 1.0 68.1