Hotspot

ycf88

SSC · 20749-21270 · 522 bp

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Pi 0.11

MarkerSeek score 77.90

Primer available yes

Label ycf88

Feature Metadata

label_name: ycf88
feature_type: gene
region: SSC
length_bp: 522
variable_sites: 57
pi: 0.11
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 77.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf88_p1	CGACCATTAACTCCATGGGG	AGCACGTGGTCGAATTCTTT	55.0	45.0	58.0	58.1	1400-1419 bp	1.0	85.8
ycf88_p2	AGGTCCGTTTGTTGCCTATC	AGCACGTGGTCGAATTCTTT	50.0	45.0	57.9	58.1	1248-1267 bp	1.0	85.6
ycf88_p3	AATGAACCCTTGTGACCACC	AGCACGTGGTCGAATTCTTT	50.0	45.0	58.0	58.1	1480 bp	0.5	59.0
ycf88_p4	TCCAACAGTTCGTGGTTCAG	AGCACGTGGTCGAATTCTTT	50.0	45.0	58.1	58.1	1501 bp	0.5	58.6
ycf88_p5	CCCAAATCGAGGTGGTCAAA	AGCACGTGGTCGAATTCTTT	50.0	45.0	58.1	58.1	1711 bp	0.5	58.3