Hotspot

secY

SSC · 26870-28117 · 1248 bp

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Pi 0.0721

MarkerSeek score 78.30

Primer available yes

Label secY

Feature Metadata

label_name: secY
feature_type: gene
region: SSC
length_bp: 1248
variable_sites: 90
pi: 0.0721
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
secY_p1	CGTGCATCAATTTGTGCCTT	TGAAACCTGAACTTCCTGCC	45.0	50.0	57.9	58.0	2073 bp	0.5	59.3
secY_p2	GGTGGAGCTGTTCGTATTGT	TGAAACCTGAACTTCCTGCC	50.0	50.0	57.9	58.0	2163 bp	0.5	59.1
secY_p3	AGGTTCAGGTGTGATTGCAG	TGAAACCTGAACTTCCTGCC	50.0	50.0	58.1	58.0	2182 bp	0.5	59.1
secY_p4	CGTGCATCAATTTGTGCCTT	ACGAGCTCCTTTGAAACCTG	45.0	50.0	57.9	58.1	2084 bp	0.5	58.5
secY_p5	CGTGCATCAATTTGTGCCTT	TTGACCACGAACAGGTAAGC	45.0	50.0	57.9	58.1	1826 bp	0.5	58.4