Hotspot

rbcR

SSC · 491-1414 · 924 bp

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Pi 0.129

MarkerSeek score 79.50

Primer available yes

Label rbcR

Feature Metadata

label_name: rbcR
feature_type: gene
region: SSC
length_bp: 924
variable_sites: 119
pi: 0.129
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcR_p1	GGTAGACGCGCTAGGTTTAG	CGTACTACCCGCACCTTTTT	55.0	50.0	57.9	58.2	1487 bp	0.5	57.5
rbcR_p2	GTAGACGCGCTAGGTTTAGG	CGTACTACCCGCACCTTTTT	55.0	50.0	57.9	58.2	1486 bp	0.5	57.5
rbcR_p3	GGTAGACGCGCTAGGTTTAG	TTTTTCGTACTACCCGCACC	55.0	50.0	57.9	58.2	1492 bp	0.5	57.4
rbcR_p4	GTAGACGCGCTAGGTTTAGG	TTTTTCGTACTACCCGCACC	55.0	50.0	57.9	58.2	1491 bp	0.5	57.4
rbcR_p5	TGAAAGATAACGGGTGTGGC	CGTACTACCCGCACCTTTTT	50.0	50.0	58.2	58.2	1513 bp	0.5	56.9