Hotspot

5S ribosomal RNA-23S ribosomal RNA

IRb · 62366-62485 · 120 bp

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Pi 0.0714

MarkerSeek score 79.10

Primer available yes

Label 5S ribosomal RNA-23S ribosomal RNA

Feature Metadata

label_name: 5S ribosomal RNA-23S ribosomal RNA
feature_type: igs
region: IRb
length_bp: 120
variable_sites: 6
pi: 0.0714
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.70
markerseek_score: 79.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
5S ribosomal RNA-23S ribosomal RNA_p1	TCGAGATGGATCAGTGTGGT	AAGCATCTAAGTGGGAAGCC	50.0	50.0	58.1	57.6	284-300 bp	1.0	81.1
5S ribosomal RNA-23S ribosomal RNA_p2	CTATCTTCCCAGGAGGTCCC	AAGCATCTAAGTGGGAAGCC	60.0	50.0	58.3	57.6	347-363 bp	1.0	79.7
5S ribosomal RNA-23S ribosomal RNA_p3	GATGGATCAGTGTGGTTCCG	AAGCATCTAAGTGGGAAGCC	55.0	50.0	58.3	57.6	280-296 bp	1.0	79.5
5S ribosomal RNA-23S ribosomal RNA_p4	GATCAGTGTGGTTCCGCTTA	AAGCATCTAAGTGGGAAGCC	50.0	50.0	57.6	57.6	276-292 bp	1.0	79.2
5S ribosomal RNA-23S ribosomal RNA_p5	ATCAGTGTGGTTCCGCTTAG	AAGCATCTAAGTGGGAAGCC	50.0	50.0	57.6	57.6	275-291 bp	1.0	79.2