Hotspot

rps6-tRNA-His

SSC · 40127-45946 · 5820 bp

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Pi 0.436

MarkerSeek score 67.00

Primer available yes

Label rps6-tRNA-His

Feature Metadata

label_name: rps6-tRNA-His
feature_type: igs
region: SSC
length_bp: 5820
variable_sites: 1412
pi: 0.436
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.56
markerseek_score: 67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps6-tRNA-His_p1	ACCTGAGGACAGAGTAGTGG	GTAATCTTAAGTGGGGGCCG	55.0	55.0	57.8	58.0	833 bp	0.5	57.9
rps6-tRNA-His_p2	ACCTGAGGACAGAGTAGTGG	TAATCTTAAGTGGGGGCCGA	55.0	50.0	57.8	58.1	832 bp	0.5	57.3
rps6-tRNA-His_p3	CCTTACCGATGGCAGAAGTT	GTAATCTTAAGTGGGGGCCG	50.0	55.0	57.6	58.0	880 bp	0.5	56.5
rps6-tRNA-His_p4	TCCTTACCGATGGCAGAAGT	GTAATCTTAAGTGGGGGCCG	50.0	55.0	58.4	58.0	881 bp	0.5	56.3
rps6-tRNA-His_p5	CCTTACCGATGGCAGAAGTT	TAATCTTAAGTGGGGGCCGA	50.0	50.0	57.6	58.1	879 bp	0.5	55.8