Hotspot

rps16-clpC

SSC · 34917-35530 · 614 bp

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Pi 0.285

MarkerSeek score 79.00

Primer available yes

Label rps16-clpC

Feature Metadata

label_name: rps16-clpC
feature_type: igs
region: SSC
length_bp: 614
variable_sites: 175
pi: 0.285
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-clpC_p1	AGACGAAAAGGTGTTACCGG	CCGTTTCAGATGCTTCAGGA	50.0	50.0	57.8	57.9	1366 bp	0.5	57.9
rps16-clpC_p2	ATTGCTCAAGCTCGACAACT	CCGTTTCAGATGCTTCAGGA	45.0	50.0	57.8	57.9	1276 bp	0.5	57.8
rps16-clpC_p3	AGACGAAAAGGTGTTACCGG	TACACTTGCTGAGCAGTGTC	50.0	50.0	57.8	57.8	1608 bp	0.5	57.5
rps16-clpC_p4	ATTGCTCAAGCTCGACAACT	TACACTTGCTGAGCAGTGTC	45.0	50.0	57.8	57.8	1518 bp	0.5	57.4
rps16-clpC_p5	AGACGAAAAGGTGTTACCGG	GCAAAATCCGACCAAGGAGA	50.0	50.0	57.8	58.2	1985 bp	0.5	57.3