Hotspot

trnW-CCA

LSC · 69844-69917 · 74 bp

Back to Pleuropterus

Pi 0.0135

MarkerSeek score 60.30

Primer available yes

Label trnW-CCA

Feature Metadata

label_name: trnW-CCA
feature_type: tRNA
region: LSC
length_bp: 74
variable_sites: 1
pi: 0.0135
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnW-CCA_p1	TGACCTCCTGTGGCTTAAAG	ATGTGATATGCATGCAGGAT	50.0	40.0	57.1	54.9	240 bp	1.0	48.8
trnW-CCA_p2	TGACCTCCTGTGGCTTAAAG	GCATGCAGGATATAGTATTGGGT	50.0	43.5	57.1	58.2	231 bp	1.0	48.3
trnW-CCA_p3	TGACCTCCTGTGGCTTAAAG	TGCATGCAGGATATAGTATTGGG	50.0	43.5	57.1	58.2	232 bp	1.0	48.2
trnW-CCA_p4	TTGACCTCCTGTGGCTTAAAG	ATGTGATATGCATGCAGGAT	47.6	40.0	57.9	54.9	241 bp	1.0	46.6
trnW-CCA_p5	GACCTCCTGTGGCTTAAAGA	ATGTGATATGCATGCAGGAT	50.0	40.0	56.8	54.9	239 bp	1.0	46.5