Hotspot

rpoC2

LSC · 17677-21753 · 4077 bp

Back to Pleuropterus

Pi 0.000245

MarkerSeek score 54.10

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4077
variable_sites: 1
pi: 0.000245
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	GGATGGTGCCACAATCTCTT	CAGGAGCTAGAGGAAATGCC	50.0	55.0	57.9	57.7	270 bp	1.0	77.2
rpoC2_p2	GGATGGTGCCACAATCTCTT	AATGCCTCTCAGGTACACCA	50.0	50.0	57.9	58.3	256 bp	1.0	76.5
rpoC2_p3	GGATGGTGCCACAATCTCTT	ATGCCTCTCAGGTACACCAA	50.0	50.0	57.9	58.3	255 bp	1.0	76.5
rpoC2_p4	GGATGGTGCCACAATCTCTT	TGCCTCTCAGGTACACCAAT	50.0	50.0	57.9	58.3	254 bp	1.0	76.5
rpoC2_p5	GGTTTGGGGACTCACAGAAA	CAGGAGCTAGAGGAAATGCC	50.0	55.0	57.6	57.7	296 bp	1.0	75.4