Hotspot

ndhD

SSC · 124808-126385 · 1578 bp

Back to Pleuropterus

Pi 0.000634

MarkerSeek score 51.20

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1578
variable_sites: 1
pi: 0.000634
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	ACAAACGTCAAACTGTCCGA	GTTTACCGACAAAAACCCGC	45.0	50.0	58.0	58.2	1685 bp	1.0	78.0
ndhD_p2	CACAAACGTCAAACTGTCCG	GTTTACCGACAAAAACCCGC	50.0	50.0	57.9	58.2	1686 bp	1.0	77.4
ndhD_p3	ACAAACGTCAAACTGTCCGA	ATGGCATGAAACAACTCGCA	45.0	45.0	58.0	58.8	1761 bp	1.0	73.9
ndhD_p4	CACAAACGTCAAACTGTCCG	ATGGCATGAAACAACTCGCA	50.0	45.0	57.9	58.8	1762 bp	1.0	73.3
ndhD_p5	ACAAACGTCAAACTGTCCGA	ACGTTCCAGAAAACTCCACT	45.0	45.0	58.0	57.0	1720 bp	1.0	71.9