Hotspot

ccsA

SSC · 123623-124588 · 966 bp

Back to Pleuropterus

Pi 0

MarkerSeek score 33.40

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 966
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 33.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	ACGATTTGTAATTGAGGGATCTCT	GTTCTCGACGAGACTTGCTT	37.5	50.0	57.8	57.9	1116 bp	1.0	45.6
ccsA_p2	ACGATTTGTAATTGAGGGATCTCT	ACCCCGTCCTGTAGTTGTAT	37.5	50.0	57.8	57.8	1066 bp	1.0	44.1
ccsA_p3	ACGATTTGTAATTGAGGGATCTCT	ATACCCCGTCCTGTAGTTGT	37.5	50.0	57.8	57.8	1068 bp	1.0	44.1
ccsA_p4	ACGATTTGTAATTGAGGGATCTC	GTTCTCGACGAGACTTGCTT	39.1	50.0	56.4	57.9	1116 bp	1.0	42.9
ccsA_p5	ACGATTTGTAATTGAGGGATCT	GTTCTCGACGAGACTTGCTT	36.4	50.0	55.2	57.9	1116 bp	1.0	41.5