Hotspot

ccsA-ndhD

SSC · 124589-124807 · 219 bp

Back to Pleuropterus

Pi 0.0457

MarkerSeek score 78.80

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 219
variable_sites: 10
pi: 0.0457
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TCTGCAATTGTGGCTTCTCT	TGGCATTTACCCAGATTTCGT	45.0	42.9	57.4	57.9	412 bp	1.0	68.7
ccsA-ndhD_p2	TCTGCAATTGTGGCTTCTCT	ACCCAGATTTCGTTCTCTCAC	45.0	47.6	57.4	57.7	404 bp	1.0	66.8
ccsA-ndhD_p3	GCAATTGTGGCTTCTCTAGGA	TGGCATTTACCCAGATTTCGT	47.6	42.9	58.0	57.9	409 bp	1.0	65.2
ccsA-ndhD_p4	TCTGCAATTGTGGCTTCTCT	CCCAGATTTCGTTCTCTCACT	45.0	47.6	57.4	57.4	403 bp	1.0	64.6
ccsA-ndhD_p5	TTCTGCAATTGTGGCTTCTCT	TGGCATTTACCCAGATTTCGT	42.9	42.9	58.1	57.9	413 bp	1.0	64.2