Hotspot

ndhF-rpl32

SSC · 121408-122481 · 1074 bp

Back to Pleuropterus

Pi 0.000931
MarkerSeek score 59.30
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1074
variable_sites
1
pi
0.000931
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 AGAAGAAGTCCTGCTCCGAT TGCTGCCCAATATCCTTTTCT 50.0 42.9 58.1 57.6 1241 bp 1.0 67.8
ndhF-rpl32_p2 AGAAGAAGTCCTGCTCCGAT GCAAAAGCTTTTAATGCTGCC 50.0 42.9 58.1 57.5 1255 bp 1.0 67.3
ndhF-rpl32_p3 AGAAGAAGTCCTGCTCCGAT AAAGCTTTTAATGCTGCCCA 50.0 40.0 58.1 56.5 1252 bp 1.0 66.8
ndhF-rpl32_p4 AGAAGAAGTCCTGCTCCGAT AAGCTTTTAATGCTGCCCAA 50.0 40.0 58.1 56.5 1251 bp 1.0 66.8
ndhF-rpl32_p5 AAGAAGAAGTCCTGCTCCGA TGCTGCCCAATATCCTTTTCT 50.0 42.9 58.4 57.6 1242 bp 1.0 66.0