Hotspot

trnV-UAC-trnM-CAU

LSC · 55181-55356 · 176 bp

Back to Pleuropterus

Pi 0.00568

MarkerSeek score 55.80

Primer available yes

Label trnV-UAC-trnM-CAU

Feature Metadata

label_name: trnV-UAC-trnM-CAU
feature_type: igs
region: LSC
length_bp: 176
variable_sites: 1
pi: 0.00568
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV-UAC-trnM-CAU_p1	CCTTGAAAAACATTGGCGCA	TTGAACCAATGACTCCTGCC	45.0	50.0	58.1	58.1	293 bp	1.0	78.5
trnV-UAC-trnM-CAU_p2	ACCAACTGAGCTATAGCCCT	TTGAACCAATGACTCCTGCC	50.0	50.0	57.8	58.1	254 bp	1.0	78.1
trnV-UAC-trnM-CAU_p3	CCTTGAAAAACATTGGCGCA	CTCCTGCCGTATGAAAGCAA	45.0	50.0	58.1	58.3	281 bp	1.0	77.1
trnV-UAC-trnM-CAU_p4	ACCAACTGAGCTATAGCCCT	CTCCTGCCGTATGAAAGCAA	50.0	50.0	57.8	58.3	242 bp	1.0	76.8
trnV-UAC-trnM-CAU_p5	CCTTGAAAAACATTGGCGCA	CCAATGACTCCTGCCGTATG	45.0	55.0	58.1	58.4	288 bp	1.0	75.9