Hotspot

rps16-trnQ-UUG

LSC · 6399-7858 · 1460 bp

Back to Pleuropterus

Pi 0

MarkerSeek score 58.80

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1460
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1532-1533 bp	1.0	78.3
rps16-trnQ-UUG_p2	TCCACATCAGATCGTGTCCT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.1	58.1	1554-1555 bp	1.0	78.3
rps16-trnQ-UUG_p3	AGATCGTGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.4	58.1	1546-1547 bp	1.0	74.3
rps16-trnQ-UUG_p4	CCACATCAGATCGTGTCCTT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.3	58.1	1553-1554 bp	1.0	74.0
rps16-trnQ-UUG_p5	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	45.0	50.0	58.1	57.3	1536-1537 bp	1.0	73.6