primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	rps16	1	AGAGCAAAAAGGGTGGGAAT	ACGTACCGACTAAACCAATGA	20	21	45.0	42.9	57.3	57.1	0.0	0.0	2.642479	5266	6398	1306-1307	1.000000	58.9
rps16_p2	rps16	2	AGAGCAAAAAGGGTGGGAAT	TGTACGTACCGACTAAACCA	20	20	45.0	45.0	57.3	56.0	0.0	22.4	2.742586	5266	6398	1309-1310	1.000000	58.1
rps16_p3	rps16	3	AGAGCAAAAAGGGTGGGAAT	CGTACCGACTAAACCAATGACT	20	22	45.0	45.5	57.3	58.2	0.0	0.0	2.860627	5266	6398	1305-1306	1.000000	57.2
rps16_p4	rps16	4	AGAGCAAAAAGGGTGGGAAT	CGTACCGACTAAACCAATGAC	20	21	45.0	47.6	57.3	56.7	0.0	0.0	2.955223	5266	6398	1305-1306	1.000000	56.4
rps16_p5	rps16	5	AGAGCAAAAAGGGTGGGAAT	TGTACGTACCGACTAAACCAA	20	21	45.0	42.9	57.3	56.7	0.0	22.4	2.976303	5266	6398	1309-1310	1.000000	56.3
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	6399	7858	1532-1533	1.000000	78.3
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	TCCACATCAGATCGTGTCCT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.1	58.1	10.3	16.9	0.223468	6399	7858	1554-1555	1.000000	78.3
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AGATCGTGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.4	58.1	0.0	16.9	0.714657	6399	7858	1546-1547	1.000000	74.3
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	CCACATCAGATCGTGTCCTT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.3	58.1	10.3	16.9	0.756413	6399	7858	1553-1554	1.000000	74.0
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	20	20	45.0	50.0	58.1	57.3	8.1	16.9	0.806797	6399	7858	1536-1537	1.000000	73.6
trnG-UCC_p1	trnG-UCC	1	CGTTGTTCGACAAAAGTTCCA	ATGGACGCTTCTCATTACGA	21	20	42.9	45.0	57.6	56.8	2.7	0.0	2.641272	10342	11139	962-962	1.000000	59.0
trnG-UCC_p2	trnG-UCC	2	CGTTGTTCGACAAAAGTTCCA	TGGACGCTTCTCATTACGAT	21	20	42.9	45.0	57.6	56.8	2.7	0.0	2.641272	10342	11139	961-961	1.000000	59.0
trnG-UCC_p3	trnG-UCC	3	TCGTTGTTCGACAAAAGTTCC	ATGGACGCTTCTCATTACGA	21	20	42.9	45.0	57.3	56.8	2.7	0.0	2.910261	10342	11139	963-963	1.000000	56.8
trnG-UCC_p4	trnG-UCC	4	TCGTTGTTCGACAAAAGTTCC	TGGACGCTTCTCATTACGAT	21	20	42.9	45.0	57.3	56.8	2.7	0.0	2.910261	10342	11139	962-962	1.000000	56.8
trnG-UCC_p5	trnG-UCC	5	CGTTGTTCGACAAAAGTTCCA	TGGACGCTTCTCATTACGATT	21	21	42.9	42.9	57.6	57.5	2.7	0.0	2.913059	10342	11139	961-961	1.000000	56.8
atpF_p1	atpF	1	TTCATCAGCTCGAAGGGTTG	AGTTTTTGAATCCATTTTCGA	20	21	50.0	28.6	57.9	52.2	0.0	0.0	6.879158	13104	14414	1513-1513	1.000000	40.1
atpF_p2	atpF	2	TTCATCAGCTCGAAGGGTTG	AGGAAATAGGTCAAGTAATACAACA	20	25	50.0	32.0	57.9	56.0	0.0	0.0	7.128303	13104	14414	1483-1483	1.000000	40.1
atpF_p3	atpF	3	TTCATCAGCTCGAAGGGTTG	AGTTTTTGAATCCATTTTCGAT	20	22	50.0	27.3	57.9	52.9	0.0	0.0	7.194864	13104	14414	1513-1513	1.000000	40.1
atpF_p4	atpF	4	TTCATCAGCTCGAAGGGTTG	AGTTTTTGAATCCATTTTCGATT	20	23	50.0	26.1	57.9	53.7	0.0	0.0	7.353388	13104	14414	1513-1513	1.000000	40.1
atpF_p5	atpF	5	GCTCGAAGGGTTGTCATGAG	AGTTTTTGAATCCATTTTCGA	20	21	55.0	28.6	58.6	52.2	0.0	0.0	7.416273	13104	14414	1506-1506	1.000000	40.1
rpoC2_p1	rpoC2	1	GGATGGTGCCACAATCTCTT	CAGGAGCTAGAGGAAATGCC	20	20	50.0	55.0	57.9	57.7	11.9	0.0	0.387765	21131	21250	270-270	1.000000	77.2
rpoC2_p2	rpoC2	2	GGATGGTGCCACAATCTCTT	AATGCCTCTCAGGTACACCA	20	20	50.0	50.0	57.9	58.3	11.9	0.4	0.478202	21131	21250	256-256	1.000000	76.5
rpoC2_p3	rpoC2	3	GGATGGTGCCACAATCTCTT	ATGCCTCTCAGGTACACCAA	20	20	50.0	50.0	57.9	58.3	11.9	0.4	0.478202	21131	21250	255-255	1.000000	76.5
rpoC2_p4	rpoC2	4	GGATGGTGCCACAATCTCTT	TGCCTCTCAGGTACACCAAT	20	20	50.0	50.0	57.9	58.3	11.9	0.4	0.478202	21131	21250	254-254	1.000000	76.5
rpoC2_p5	rpoC2	5	GGTTTGGGGACTCACAGAAA	CAGGAGCTAGAGGAAATGCC	20	20	50.0	55.0	57.6	57.7	0.0	0.0	0.611298	21131	21250	296-296	1.000000	75.4
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	58.3	58.0	0.0	0.0	0.269359	33217	34130	1017-1017	1.000000	77.9
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.287509	33217	34130	996-996	1.000000	77.8
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	GCTGCCTCCTTGAAAGAGAG	CCATGGCGTTACTCTACCAC	20	20	55.0	55.0	58.3	58.1	0.0	0.0	0.327456	33217	34130	996-996	1.000000	77.5
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	TGTCCTGAACCACTAGACGA	CCATGGCGTTACTCTACCAC	20	20	50.0	55.0	57.7	58.1	0.0	0.0	0.345606	33217	34130	975-975	1.000000	77.3
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	GCTGCCTCCTTGAAAGAGAG	TGGCGTTACTCTACCACTGA	20	20	55.0	50.0	58.3	58.1	0.0	0.0	0.349611	33217	34130	993-993	1.000000	77.3
trnV-UAC-trnM-CAU_p1	trnV-UAC-trnM-CAU	1	CCTTGAAAAACATTGGCGCA	TTGAACCAATGACTCCTGCC	20	20	45.0	50.0	58.1	58.1	10.8	0.0	0.227033	55181	55356	293-293	1.000000	78.5
trnV-UAC-trnM-CAU_p2	trnV-UAC-trnM-CAU	2	ACCAACTGAGCTATAGCCCT	TTGAACCAATGACTCCTGCC	20	20	50.0	50.0	57.8	58.1	21.2	0.0	0.272816	55181	55356	254-254	1.000000	78.1
trnV-UAC-trnM-CAU_p3	trnV-UAC-trnM-CAU	3	CCTTGAAAAACATTGGCGCA	CTCCTGCCGTATGAAAGCAA	20	20	45.0	50.0	58.1	58.3	10.8	0.0	0.398895	55181	55356	281-281	1.000000	77.1
trnV-UAC-trnM-CAU_p4	trnV-UAC-trnM-CAU	4	ACCAACTGAGCTATAGCCCT	CTCCTGCCGTATGAAAGCAA	20	20	50.0	50.0	57.8	58.3	21.2	0.0	0.444678	55181	55356	242-242	1.000000	76.8
trnV-UAC-trnM-CAU_p5	trnV-UAC-trnM-CAU	5	CCTTGAAAAACATTGGCGCA	CCAATGACTCCTGCCGTATG	20	20	45.0	55.0	58.1	58.4	10.8	0.0	0.550311	55181	55356	288-288	1.000000	75.9
rbcL_p1	rbcL	1	ACGCCTAATTCATGTCGAGT	AGTAAAAGATTGAGCCGAGT	20	20	45.0	40.0	57.0	54.4	0.0	0.0	4.570755	58411	59859	1553-1553	1.000000	43.4
rbcL_p2	rbcL	2	ACGCCTAATTCATGTCGAGT	AGTAAAAGATTGAGCCGAGTT	20	21	45.0	38.1	57.0	55.2	0.0	0.0	4.721730	58411	59859	1553-1553	1.000000	42.2
rbcL_p3	rbcL	3	AACGCCTAATTCATGTCGAGT	AGTAAAAGATTGAGCCGAGT	21	20	42.9	40.0	57.8	54.4	0.0	0.0	4.856235	58411	59859	1554-1554	1.000000	41.2
rbcL_p4	rbcL	4	ACGCCTAATTCATGTCGAGT	AGTAAAAGATTGAGCCGAGTTT	20	22	45.0	36.4	57.0	56.0	0.0	0.0	4.949371	58411	59859	1553-1553	1.000000	40.4
rbcL_p5	rbcL	5	AACGCCTAATTCATGTCGAGT	AGTAAAAGATTGAGCCGAGTT	21	21	42.9	38.1	57.8	55.2	0.0	0.0	5.007209	58411	59859	1554-1554	1.000000	40.0
rbcL-accD_p1	rbcL-accD	1	AATGGAGTCCTGAGCTAGCT	TAGCCCACACCTATGTTCGA	20	20	50.0	50.0	57.8	58.1	19.4	0.0	0.317831	59860	60344	628-628	1.000000	77.8
rbcL-accD_p2	rbcL-accD	2	TCGTAAAGCTGCCAAATGGA	TAGCCCACACCTATGTTCGA	20	20	45.0	50.0	57.8	58.1	3.0	0.0	0.337368	59860	60344	642-642	1.000000	77.7
rbcL-accD_p3	rbcL-accD	3	CTGCCAAATGGAGTCCTGAG	TAGCCCACACCTATGTTCGA	20	20	55.0	50.0	58.3	58.1	11.3	0.0	0.396996	59860	60344	634-634	1.000000	77.2
rbcL-accD_p4	rbcL-accD	4	CGTAAAGCTGCCAAATGGAG	TAGCCCACACCTATGTTCGA	20	20	50.0	50.0	57.5	58.1	3.0	0.0	0.694338	59860	60344	641-641	1.000000	74.8
rbcL-accD_p5	rbcL-accD	5	GCCGCTTGTGAAGTATGGAA	TAGCCCACACCTATGTTCGA	20	20	50.0	50.0	58.6	58.1	0.0	0.0	0.695720	59860	60344	608-608	1.000000	74.8
trnW-CCA_p1	trnW-CCA	1	TGACCTCCTGTGGCTTAAAG	ATGTGATATGCATGCAGGAT	20	20	50.0	40.0	57.1	54.9	0.0	26.7	3.936388	69844	69917	240-240	1.000000	48.8
trnW-CCA_p2	trnW-CCA	2	TGACCTCCTGTGGCTTAAAG	GCATGCAGGATATAGTATTGGGT	20	23	50.0	43.5	57.1	58.2	0.0	14.2	4.010808	69844	69917	231-231	1.000000	48.3
trnW-CCA_p3	trnW-CCA	3	TGACCTCCTGTGGCTTAAAG	TGCATGCAGGATATAGTATTGGG	20	23	50.0	43.5	57.1	58.2	0.0	18.5	4.011967	69844	69917	232-232	1.000000	48.2
trnW-CCA_p4	trnW-CCA	4	TTGACCTCCTGTGGCTTAAAG	ATGTGATATGCATGCAGGAT	21	20	47.6	40.0	57.9	54.9	0.0	26.7	4.217548	69844	69917	241-241	1.000000	46.6
trnW-CCA_p5	trnW-CCA	5	GACCTCCTGTGGCTTAAAGA	ATGTGATATGCATGCAGGAT	20	20	50.0	40.0	56.8	54.9	0.0	26.7	4.229356	69844	69917	239-239	1.000000	46.5
ycf1_p1	ycf1	1	ACTGGTTCTATGCGTGTTCA	CCGCCCCTTTTCCATTTCAT	20	20	45.0	50.0	57.2	58.8	0.0	0.0	1.637467	115765	115884	297-297	1.000000	67.7
ycf1_p2	ycf1	2	TCACTGGTTCTATGCGTGTT	CCGCCCCTTTTCCATTTCAT	20	20	45.0	50.0	57.2	58.8	0.0	0.0	1.637467	115765	115884	299-299	1.000000	67.7
ycf1_p3	ycf1	3	ACTGGTTCTATGCGTGTTCA	TTCGTTCCATTTCCGTCTGC	20	20	45.0	50.0	57.2	58.8	0.0	0.0	1.678332	115765	115884	322-322	1.000000	67.3
ycf1_p4	ycf1	4	TCACTGGTTCTATGCGTGTT	TTCGTTCCATTTCCGTCTGC	20	20	45.0	50.0	57.2	58.8	0.0	0.0	1.678332	115765	115884	324-324	1.000000	67.3
ycf1_p5	ycf1	5	TCTATGCGTGTTCAAAGACGT	CCGCCCCTTTTCCATTTCAT	21	20	42.9	50.0	58.0	58.8	13.0	0.0	1.827051	115765	115884	291-291	1.000000	66.2
ndhF-rpl32_p1	ndhF-rpl32	1	AGAAGAAGTCCTGCTCCGAT	TGCTGCCCAATATCCTTTTCT	20	21	50.0	42.9	58.1	57.6	0.0	0.0	1.527630	121408	122481	1241-1241	1.000000	67.8
ndhF-rpl32_p2	ndhF-rpl32	2	AGAAGAAGTCCTGCTCCGAT	GCAAAAGCTTTTAATGCTGCC	20	21	50.0	42.9	58.1	57.5	0.0	23.4	1.601060	121408	122481	1255-1255	1.000000	67.3
ndhF-rpl32_p3	ndhF-rpl32	3	AGAAGAAGTCCTGCTCCGAT	AAAGCTTTTAATGCTGCCCA	20	20	50.0	40.0	58.1	56.5	0.0	5.7	1.660540	121408	122481	1252-1252	1.000000	66.8
ndhF-rpl32_p4	ndhF-rpl32	4	AGAAGAAGTCCTGCTCCGAT	AAGCTTTTAATGCTGCCCAA	20	20	50.0	40.0	58.1	56.5	0.0	6.1	1.660540	121408	122481	1251-1251	1.000000	66.8
ndhF-rpl32_p5	ndhF-rpl32	5	AAGAAGAAGTCCTGCTCCGA	TGCTGCCCAATATCCTTTTCT	20	21	50.0	42.9	58.4	57.6	0.0	0.0	1.753159	121408	122481	1242-1242	1.000000	66.0
ccsA_p1	ccsA	1	ACGATTTGTAATTGAGGGATCTCT	GTTCTCGACGAGACTTGCTT	24	20	37.5	50.0	57.8	57.9	0.0	26.5	4.293831	123623	124588	1116-1116	1.000000	45.6
ccsA_p2	ccsA	2	ACGATTTGTAATTGAGGGATCTCT	ACCCCGTCCTGTAGTTGTAT	24	20	37.5	50.0	57.8	57.8	0.0	0.0	4.483431	123623	124588	1066-1066	1.000000	44.1
ccsA_p3	ccsA	3	ACGATTTGTAATTGAGGGATCTCT	ATACCCCGTCCTGTAGTTGT	24	20	37.5	50.0	57.8	57.8	0.0	0.0	4.483431	123623	124588	1068-1068	1.000000	44.1
ccsA_p4	ccsA	4	ACGATTTGTAATTGAGGGATCTC	GTTCTCGACGAGACTTGCTT	23	20	39.1	50.0	56.4	57.9	0.0	26.5	4.638276	123623	124588	1116-1116	1.000000	42.9
ccsA_p5	ccsA	5	ACGATTTGTAATTGAGGGATCT	GTTCTCGACGAGACTTGCTT	22	20	36.4	50.0	55.2	57.9	0.0	26.5	4.815923	123623	124588	1116-1116	1.000000	41.5
ccsA-ndhD_p1	ccsA-ndhD	1	TCTGCAATTGTGGCTTCTCT	TGGCATTTACCCAGATTTCGT	20	21	45.0	42.9	57.4	57.9	7.4	0.0	1.654305	124589	124807	412-412	1.000000	68.7
ccsA-ndhD_p2	ccsA-ndhD	2	TCTGCAATTGTGGCTTCTCT	ACCCAGATTTCGTTCTCTCAC	20	21	45.0	47.6	57.4	57.7	7.4	0.0	1.894679	124589	124807	404-404	1.000000	66.8
ccsA-ndhD_p3	ccsA-ndhD	3	GCAATTGTGGCTTCTCTAGGA	TGGCATTTACCCAGATTTCGT	21	21	47.6	42.9	58.0	57.9	7.4	0.0	2.089049	124589	124807	409-409	1.000000	65.2
ccsA-ndhD_p4	ccsA-ndhD	4	TCTGCAATTGTGGCTTCTCT	CCCAGATTTCGTTCTCTCACT	20	21	45.0	47.6	57.4	57.4	7.4	0.0	2.175448	124589	124807	403-403	1.000000	64.6
ccsA-ndhD_p5	ccsA-ndhD	5	TTCTGCAATTGTGGCTTCTCT	TGGCATTTACCCAGATTTCGT	21	21	42.9	42.9	58.1	57.9	7.4	0.0	2.212676	124589	124807	413-413	1.000000	64.2
ndhD_p1	ndhD	1	ACAAACGTCAAACTGTCCGA	GTTTACCGACAAAAACCCGC	20	20	45.0	50.0	58.0	58.2	0.0	0.0	0.255363	124808	126385	1685-1685	1.000000	78.0
ndhD_p2	ndhD	2	CACAAACGTCAAACTGTCCG	GTTTACCGACAAAAACCCGC	20	20	50.0	50.0	57.9	58.2	0.0	0.0	0.327958	124808	126385	1686-1686	1.000000	77.4
ndhD_p3	ndhD	3	ACAAACGTCAAACTGTCCGA	ATGGCATGAAACAACTCGCA	20	20	45.0	45.0	58.0	58.8	0.0	0.0	0.766288	124808	126385	1761-1761	1.000000	73.9
ndhD_p4	ndhD	4	CACAAACGTCAAACTGTCCG	ATGGCATGAAACAACTCGCA	20	20	50.0	45.0	57.9	58.8	0.0	0.0	0.838883	124808	126385	1762-1762	1.000000	73.3
ndhD_p5	ndhD	5	ACAAACGTCAAACTGTCCGA	ACGTTCCAGAAAACTCCACT	20	20	45.0	45.0	58.0	57.0	0.0	0.0	1.015393	124808	126385	1720-1720	1.000000	71.9
rps15_p1	rps15	1	GCCAGTGACAGCTTCGAATA	TGGATAAATAGCTGCGCACA	20	20	50.0	45.0	58.0	57.7	0.0	5.7	0.334113	132480	132743	1070-1157	1.000000	82.4
rps15_p2	rps15	2	GCCAGTGACAGCTTCGAATA	GGTGGATAAATAGCTGCGCA	20	20	50.0	50.0	58.0	58.4	0.0	11.0	0.416949	132480	132743	1072-1159	1.000000	81.7
rps15_p3	rps15	3	TAAGGTAAATCGGCAGCGAC	TGGATAAATAGCTGCGCACA	20	20	50.0	45.0	58.1	57.7	0.0	5.7	0.393401	132480	132743	1126-1213	1.000000	81.6
rps15_p4	rps15	4	TCTTCTCCGCCAATAATGCC	TGGATAAATAGCTGCGCACA	20	20	50.0	45.0	58.0	57.7	0.0	5.7	0.356650	132480	132743	1258-1345	1.000000	81.4
rps15_p5	rps15	5	TTTATTGCCTCTTCTCCGCC	TGGATAAATAGCTGCGCACA	20	20	50.0	45.0	58.0	57.7	0.0	5.7	0.362355	132480	132743	1267-1354	1.000000	81.4
rps15-ycf1_p1	rps15-ycf1	1	AAGTCGTTGACGTTTTCCCA	GGATCCAATGCTAATCCGGG	20	20	45.0	55.0	58.0	58.2	3.2	7.0	0.200618	132744	133035	1058-1145	1.000000	83.5
rps15-ycf1_p2	rps15-ycf1	2	AGTCGTTGACGTTTTCCCAA	GGATCCAATGCTAATCCGGG	20	20	45.0	55.0	58.0	58.2	3.2	7.0	0.200618	132744	133035	1057-1144	1.000000	83.5
rps15-ycf1_p3	rps15-ycf1	3	AAGTCGTTGACGTTTTCCCA	GATTATGCGCTGTCCGTACT	20	20	45.0	50.0	58.0	57.9	3.2	9.7	0.162668	132744	133035	1150-1237	1.000000	83.4
rps15-ycf1_p4	rps15-ycf1	4	AGTCGTTGACGTTTTCCCAA	GATTATGCGCTGTCCGTACT	20	20	45.0	50.0	58.0	57.9	3.2	9.7	0.162668	132744	133035	1149-1236	1.000000	83.4
rps15-ycf1_p5	rps15-ycf1	5	TAAGCCAGAAGTCGTTGACG	GATTATGCGCTGTCCGTACT	20	20	50.0	50.0	57.9	57.9	0.0	9.7	0.195990	132744	133035	1158-1245	1.000000	83.1