Hotspot

rpl16

LSC · 76482-77931 · 1450 bp

Back to Pleuropogon

Pi 0.0156

MarkerSeek score 46.80

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1450
variable_sites: 36
pi: 0.0156
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 46.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TTTCTAGAGGCATTTGGGGC	CTTCGCATTCTGTCCAGTGA	50.0	50.0	57.9	57.9	1846-1865 bp	1.0	81.5
rpl16_p2	TCAGCAATAGTGTCCTTGCC	CTTCGCATTCTGTCCAGTGA	50.0	50.0	57.9	57.9	2211-2225 bp	1.0	81.4
rpl16_p3	TCAGCAATAGTGTCCTTGCC	TCTTCGCATTCTGTCCAGTG	50.0	50.0	57.9	57.9	2212-2226 bp	1.0	81.4
rpl16_p4	TCAGCAATAGTGTCCTTGCC	TGTCTTCGCATTCTGTCCAG	50.0	50.0	57.9	57.9	2214-2228 bp	1.0	81.4
rpl16_p5	TCAGCAATAGTGTCCTTGCC	GTCTTCGCATTCTGTCCAGT	50.0	50.0	57.9	57.9	2213-2227 bp	1.0	81.4