Hotspot

rps11-rpl36

LSC · 74641-74820 · 180 bp

Back to Pleuropogon

Pi 0.0361

MarkerSeek score 67.50

Primer available yes

Label rps11-rpl36

Feature Metadata

label_name: rps11-rpl36
feature_type: igs
region: LSC
length_bp: 180
variable_sites: 11
pi: 0.0361
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11-rpl36_p1	ACGCACTCTCTTACGTGAAC	AGCATAAACAAAGACAGGGGT	50.0	42.9	58.0	57.5	243-248 bp	1.0	73.6
rps11-rpl36_p2	TCTACGTGCACTCTTCCGTA	AGCATAAACAAAGACAGGGGT	50.0	42.9	58.2	57.5	294-299 bp	1.0	71.4
rps11-rpl36_p3	CGTGCACTCTTCCGTAAACT	AGCATAAACAAAGACAGGGGT	50.0	42.9	58.2	57.5	290-295 bp	1.0	71.2
rps11-rpl36_p4	ATTCTACGTGCACTCTTCCG	AGCATAAACAAAGACAGGGGT	50.0	42.9	57.7	57.5	296-301 bp	1.0	70.6
rps11-rpl36_p5	ACGTGCACTCTTCCGTAAAC	AGCATAAACAAAGACAGGGGT	50.0	42.9	58.5	57.5	291-296 bp	1.0	68.9