Hotspot

psbE-petG

LSC · 61063-62493 · 1431 bp

Back to Pleuropogon

Pi 0.0233

MarkerSeek score 69.90

Primer available yes

Label psbE-petG

Feature Metadata

label_name: psbE-petG
feature_type: igs
region: LSC
length_bp: 1431
variable_sites: 55
pi: 0.0233
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 69.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petG_p1	CAAAAGAACGTTCTCCCGTG	ACGCAGTCACGAATAATCCC	50.0	50.0	57.3	58.1	1460-1568 bp	1.0	81.1
psbE-petG_p2	CAGCAAAAGAACGTTCTCCC	ACGCAGTCACGAATAATCCC	50.0	50.0	57.3	58.1	1463-1571 bp	1.0	80.9
psbE-petG_p3	AGCAAAAGAACGTTCTCCCG	ACGCAGTCACGAATAATCCC	50.0	50.0	58.8	58.1	1462-1570 bp	1.0	80.2
psbE-petG_p4	CAAAAGAACGTTCTCCCGTG	CAGTCACGAATAATCCCGCT	50.0	50.0	57.3	57.8	1457-1565 bp	1.0	79.8
psbE-petG_p5	CAGCAAAAGAACGTTCTCCC	CAGTCACGAATAATCCCGCT	50.0	50.0	57.3	57.8	1460-1568 bp	1.0	79.6