Hotspot

ndhC-trnV

LSC · 50267-51074 · 808 bp

Back to Pleuropogon

Pi 0.0256

MarkerSeek score 77.90

Primer available yes

Label ndhC-trnV

Feature Metadata

label_name: ndhC-trnV
feature_type: igs
region: LSC
length_bp: 808
variable_sites: 34
pi: 0.0256
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 77.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV_p1	CTAACCGGGGCTAAAAGTCC	GGTTCGAATCCGTATAGCCC	55.0	55.0	58.0	57.9	854-972 bp	1.0	86.0
ndhC-trnV_p2	CTAACCGGGGCTAAAAGTCC	CGGTTCGAATCCGTATAGCC	55.0	55.0	58.0	58.3	855-973 bp	1.0	84.2
ndhC-trnV_p3	TAACCGGGGCTAAAAGTCCT	GGTTCGAATCCGTATAGCCC	50.0	55.0	58.3	57.9	853-971 bp	1.0	83.6
ndhC-trnV_p4	CACTAACCGGGGCTAAAAGT	GGTTCGAATCCGTATAGCCC	50.0	55.0	57.5	57.9	856-974 bp	1.0	82.4
ndhC-trnV_p5	TAACCGGGGCTAAAAGTCCT	CGGTTCGAATCCGTATAGCC	50.0	55.0	58.3	58.3	854-972 bp	1.0	81.8